About Microcourses

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We are a team of light microscopists from core facilities at Harvard Medical School. We teach microscopy at HMS, and run the Quantitative Imaging: From Acquisition to Analysis course at Cold Spring Harbor Laboratory. On this channel, you can find practical instructional videos on various microscopy topics, pretty movies collected on microscopes in our cores, and some random fun stuff. Enjoy!

This channel was created by Jennifer Waters, Director of the Nikon Imaging Center at Harvard Medical School & Chan Zuckerberg Initiative Imaging Scientist. (@JenCWaters on Twitter)

Links to our websites below

Chromatic Shifts

This microcourse covers common sources of shifts between images of different wavelengths (e.g., when imaging multiple fluorophores in the same specimen. Some additional info: Waters, J.C. (2009) Accuracy and precision in quantitative fluorescence microscopy. J. Cell Biol. 185, 1135–1148 Ross, S.T.…

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The Confocal Principle

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Fluorescence Filters: Imaging Multiple Fluorophores & Dealing with Bleed-through

*This video walks you through best practices for imaging more than one fluorophore, including critical controls experiments, why bleed-through can occur when imaging multiple fluorophores and various strategies for reducing bleed-through. Enjoy! * Visit FPbase.org/microscopes to generate a custom …

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